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Selective Plane Illumination Microscopy SPIM
Selective Plane Illumination Microscopy (SPIM) is a powerful imaging technique used in biological research. It offers 3D imaging with high resolution and contrast, making it a valuable tool for studying dynamic processes within living organisms.
Advantages of SPIM
- Wide field of view: Allows for imaging of large sample volumes.
- High resolution: Provides detailed images with submicron resolution.
- Minimal photobleaching: Preserves sample integrity during imaging.
- Low phototoxicity: Suitable for live cell imaging.
Applications of SPIM
- Embryonic development: Studying organ and tissue formation in early stages.
- Neuroscience: Visualizing neural circuits and synaptic activity.
- Cancer research: Imaging tumor growth and metastasis dynamics.
- Developmental biology: Monitoring morphogenesis and tissue remodeling.
Technical Principle
SPIM operates by selectively illuminating a single plane of the sample at a time. Light is focused onto the sample through an objective lens located perpendicular to the imaging axis. Fluorescence emitted from the illuminated plane is collected by a detection system, resulting in a cross-sectional image. By scanning the illumination plane through the sample volume, a 3D reconstruction of the specimen can be obtained.
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